| Böttcher, C; v. Roepenack-Lahaye, E; Schmidt, J; Schmotz, C; Neumann, S; Scheel, D; Clemens, S: Metabolome Analysis of Biosynthetic Mutants Reveals Diversity of Metabolic Changes and Allows Identification of a Large Number of New Compounds in Arabidopsis thaliana, Plant Physiology, 147, 2107-2120 (2008), doi:10.1104/pp.108.117754 | |
| Abstract: Metabolomics is facing a major challenge: the lack of knowledge about metabolites present in a given biological system. Thus, large-scale discovery of metabolites is considered an essential step towards a better understanding of plant metabolism. We show here that the application of a metabolomics approach generating structural information for the analysis of A. thaliana mutants allows efficient cataloging of metabolites. 56 % of the features that showed significant differences in abundance between seeds of wild type, tt4 and tt5 mutant plants could be annotated. Seventy five compounds were structurally characterized, 21 of which could be identified. About 40 compounds had not been known from A. thaliana before. Also, the high resolution analysis revealed an unanticipated expansion of metabolic conversions upstream of biosynthetic blocks. Deficiency in chalcone synthase results in the increased seed-specific biosynthesis of a range of phenolic choline esters. Similarly, a lack of chalcone isomerase activity leads to the accumulation of various naringenin chalcone derivatives. Furthermore, our data provide insight into the connection between p-coumaroyl-CoA-dependent pathways. Lack of flavonoid biosynthesis results in elevated synthesis not only of p-coumarate derived choline esters but also of sinapate-derived metabolites. However, sinapoylcholine is not the only accumulating end product. Instead, we observed specific and sophisticated changes in the complex pattern of sinapate-derivatives. |
Upcoming ...
Upcoming ...
| BayCEER Colloquium: |
|---|
| Th. 2024-04-25 Perspectives and challenges in the restoration and conservation of two isolated habitats: gypsum and cliffs |
| BayCEER Short Courses: |
| We. 2024-04-24 Mobile Film Making Workshop (for PhDs/PostDosc/Profs of BayCEER) |
| Fr. 2024-04-26 Mobile Film Making Workshop (for students of BayCEER) |
| Ecological-Botanical Garden: |
| Fr. 2024-04-19 now Führung | Gesteine im Ökologisch-Botanischen Garten |
| Su. 2024-04-21 Führung | Den ÖBG kennenlernen: Allgemeine Gartenführung |