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TZUNTIL:20180325T010000Z
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DTSTART:20151025T030000
TZOFFSETFROM:+0200
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RDATE:20161030T030000
RDATE:20171029T030000
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UID:www.bayceer.uni-bayreuth.de-bayceer-t135820id
DTSTAMP:20260524T130713Z
DESCRIPTION:Herbarium genomics is a promising field\, as next generation se
 quencing approaches are well-suited to deal with the usually fragmented na
 ture of archival DNA. We show that routine assembly of plastome sequences 
 from herbarium specimens is feasible\, from total DNA extracts and apparen
 tly only weakly depending on specimen age. We use genome skimming and an a
 utomated bioinformatics pipeline\, comprising Iterative Organelle Genome A
 ssembly\, that assembles paired-end reads into a series of candidate assem
 blies\, the best one of which is selected based on assembly likelihood est
 imation. Using a range of herbarium specimens\, from 12 different Angiospe
 rm families and some of which up to 146 years old\, we show that routine p
 lastome sequencing from herbarium specimens using genome skimming is feasi
 ble and cost-effective\, and can be performed with highly limited sample d
 estruction. Comparison of fresh DNA with both old and young herbarium from
  the same tree individuals shows that post-mortem damage in herbarium DNA 
 is negligible.\n \n*** invited by Sigrid Liede-Schumann and Ulrich Meve\, 
 Plant Systematics
DTSTART;TZID=Europe/Berlin:20160616T120000
DTEND;TZID=Europe/Berlin:20160616T133000
LOCATION:H6\, GEO
SUMMARY:Freek T. Bakker (PhD)\, Biosystematics Group\, Wageningen Universit
 y\, NL (Homepage): Herbarium genomics\, skimming & plastomics
TRANSP:TRANSPARENT
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