Methods applied in our department
Anoxic
cultivation methods (cultivation of acetogens, methanogens,
sulfate reducers)
O2-free chambers (for
handling
O2-sensitive cultures and
purifying O2-sensitive
enzymes)
UV-Vis Spectroscopy (for enzyme assays and membrane analyses)
High performance liquid chromatography (HPLC) (alcohols, sugars, amino acids)
Gas chromatography (GC) (H2, N2O, CH4, CO2)
Micromanipulator controlled microelectrodes (O2, N2O, pH)
Preparative centrifugation (harvesting of microorganisms)
Ultracentrifugation (membrane or DNA preparations)
Liquid scintillation (for 14C and 35S tracer studies)
DGGE and SDS Electrophoresis
DNA-Sequencer (Genesequencing, T-RFLP)
Thermocycler for PCR (amplification of genes)
Phylogenetic analysis (16S rRNA or functional genes)
Fluorescence in situ hybridization (FISH)
Phospho- and fluorescence imager (for slot blot hybridization of extracted RNA)
UV-Vis Spectroscopy (for enzyme assays and membrane analyses)
High performance liquid chromatography (HPLC) (alcohols, sugars, amino acids)
Gas chromatography (GC) (H2, N2O, CH4, CO2)
Micromanipulator controlled microelectrodes (O2, N2O, pH)
Preparative centrifugation (harvesting of microorganisms)
Ultracentrifugation (membrane or DNA preparations)
Liquid scintillation (for 14C and 35S tracer studies)
DGGE and SDS Electrophoresis
DNA-Sequencer (Genesequencing, T-RFLP)
Thermocycler for PCR (amplification of genes)
Phylogenetic analysis (16S rRNA or functional genes)
Fluorescence in situ hybridization (FISH)
Phospho- and fluorescence imager (for slot blot hybridization of extracted RNA)



