Relaxed eddy accumulation is still applied in ecosystem sciences for measuring trace gas fluxes. On managed grasslands, the length of time between management events and the application of relaxed eddy accumulation has an essential influence on the determination of the proportionality factor b and thereby on the resulting flux. In this study this effect is discussed for the first time. Also, scalar similarity between proxy scalars and scalars of  interest is affected until the ecosystem has completely recovered. Against this background, CO₂ fluxes were continuously measured and ¹³CO₂ isofluxes were determined with a high measurement precision on two representative days in summer 2010.

Moreover, a common method for the partitioning of the net ecosystem exchange into assimilation and respiration based on temperature and light response was compared with an isotopic approach directly based on the isotope discrimination of the biosphere. This approach worked well on the grassland site and could enhance flux partitioning results by better reproducing the environmental conditions.