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| Braus-Stromeyer, S; Schnappauf, G; Braus, GH; Gößner, A; Drake, HL: Carbonic Anhydrase in Acetobacterium woodii and Other Acetogenic Bacteria, J. Bacteriology, 179/22, 7197-7200 (1997) | |
Abstract:
Acetobacterium woodii, Acetohalobium arabaticum, Clostridium formicoaceticum, and Sporomusa silvacetica contained carbonic anhydrase (CA). Minimal to no CA activity was detected in Moorella thermoautotrophica, Moorella thermoacetica subsp. "pratumsolum", Sporomusa termitida, Thermoanaerobacter kivui. A. woodii contained the highest CA specific activity of the acetogens evaluated, with activities approximating 14 units mg (protein)-1 in cell extracts of either glucose- or H2-CO2-cultivated cells. CA of A. woodii was cytoplasmic and was purified approximately 300-fold to a specific activity of 5,236 units mg (protein)-1. Intracellular acetate concentrations inhibited CA activity of A. woodii by 50-85%, indicating that intracellular acetate may affect in situ CA activity. |
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