Quaroni, L; Obst, M; Nowak, M; Zobi, F: Three-Dimensional Mid-Infrared Tomographic Imaging of Endogenous and Exogenous Molecules in a Single Intact Cell with Subcellular Resolution, Angewandte Chemie International Edition, 54(1), 318-322 (2015), online: 2014-11-13, doi:10.1002/anie.201407728 [Link]
Abstract:

Microscopy in the mid-infrared spectral range provides detailed chemical information on a sample at moderate spatial resolution and is being used increasingly in the characterization of biological entities as challenging as single cells. However, a conventional cellular 2D imaging measurement is limited in its ability to associate specific compositional information to subcellular structures because of the interference from the complex topography of the sample. Herein we provide a method and protocols that overcome this challenge in which tilt-series infrared tomography is used with a standard benchtop infrared microscope. This approach gives access to the quantitative 3D distribution of molecular components based on the intrinsic contrast provided by the sample. We demonstrate the method by quantifying the distribution of an exogenous metal carbonyl complex through- out the cell and by reporting changes in its coordination sphere in different locations in the cell.

Upcoming ...




BayCEER Colloquium:
Th. 2020-01-30
BayCEER Mitgliederversammlung
Ecological-Botanical Garden:
Su. 2020-02-02
Mehr als Sonne und Strand: Pflanzen der Karibik
Geographisches Kolloquium:
Tu. 2020-01-28
Adivasis in the Anthropocene - What an Environmental History of Eastern India can tell us about the global agricultural and food crisis
Naturwissenschaftliche Gesellschaft Bayreuth:
Mo. 2020-01-27
Das Insekten- und Vogelsterben vor dem Hintergrund der Natur- und Kulturlandschaftsgeschichte – und wie wir es überwinden können
SFB Mikroplastik:
Mo. 2020-01-27
Wissenschaftskommunikation: Nachhaltigkeit und Mikroplastik
BayCEER Blog
13.01.2020
Why Science Communication?
24.05.2019
Stoichiometric controls of C and N cycling
07.05.2019
Flying halfway across the globe to dig in the dirt – a research stay in Bloomington, USA
07.05.2019
EGU – interesting research and free coffee
RSS Blog as RSS Feed
Weather research site
Luftdruck (356m): 978.6 hPa
Lufttemperatur: -0.7 °C
Niederschlag: 0.0 mm/24h
Sonnenschein: 7 h/d
Wind (Höhe 17m): 4.0 km/h
Wind (Max.): 10.8 km/h
Windrichtung: SO

...more
Globalstrahlung: 0 W/m²
Wind (Höhe 32m): 7.3 km/h

...more
This site makes use of cookies More information