Obst, M; Gasser, P; Mavrocordatos, D; Dittrich, M: TEM-specimen preparation of cell/mineral interfaces by Focused Ion Beam milling, American Mineralogist, 90(8-9), 1270-1277 (2005), doi:10.2138/am.2005.1743 [Link]
Abstract:

Picocyanobacteria were found to play an important role in calcite precipitation in oligotrophic lakes. In this study, investigations on the interface between cyanobacteria and attached biogenic calcite crystals have been performed to gain further insights into the mechanisms of nucleation of these precipitates. Ultramicrotomy, the conventional preparation technique of thin sections for Transmission Electron Microscopy (TEM) investigations, often fails when working on heterogeneous samples containing soft organic material and hard minerals. Thus, in this study the thin sections were prepared using Focused Ion Beam (FIB) milling. This approach is usually applied in material sciences but until recently was not very common in environmental research. Different analytical TEM methods like Electron Spectroscopic Imaging (ESI) and Electron Energy Loss Spectrometry (EELS) were used to test the suitability of FIB-milling for the preparation of organic/inorganic interface specimens. With this approach we were able to analyze both organic and the inorganic phases of the same sample. Elemental maps of the samples were also calculated. By analyzing the structure of the C K-absorption edge, the different bonding forms of the organic carbon cell and the inorganic carbon of the crystal could be clearly distinguished.

Upcoming ...



BayCEER Colloquium:
Th. 2020-01-23
Understanding biodiversity dynamics – from human dominated systems to the fossil record
Th. 2020-01-30
BayCEER Mitgliederversammlung
Ecological-Botanical Garden:
Su. 2020-02-02
Mehr als Sonne und Strand: Pflanzen der Karibik
Geographisches Kolloquium:
Tu. 2020-01-28
Adivasis in the Anthropocene - What an Environmental History of Eastern India can tell us about the global agricultural and food crisis
SFB Mikroplastik:
Mo. 2020-01-27
Wissenschaftskommunikation: Nachhaltigkeit und Mikroplastik
BayCEER Blog
13.01.2020
Why Science Communication?
24.05.2019
Stoichiometric controls of C and N cycling
07.05.2019
Flying halfway across the globe to dig in the dirt – a research stay in Bloomington, USA
07.05.2019
EGU – interesting research and free coffee
RSS Blog as RSS Feed
Weather research site
Luftdruck (356m): 991.8 hPa
Lufttemperatur: -4.2 °C
Niederschlag: 0.0 mm/24h
Sonnenschein: <1 h/d
Wind (Höhe 17m): 5.1 km/h
Wind (Max.): 7.9 km/h
Windrichtung: SW

...more
Globalstrahlung: 99 W/m²
Lufttemperatur: -5.2 °C
Niederschlag: 0.0 mm/24h
Sonnenschein: <1 h/d
Wind (Höhe 32m): 6.8 km/h

...more
This site makes use of cookies More information