Schmid, G; Zeitvogel, F; Hao, L; Ingino, P; Floetenmeyer, M; Stierhof, Y-D; Schroeppel, B; Burkhardt, CJ; Kappler, A; Obst, M: 3-D analysis of bacterial cell-(iron)mineral aggregates formed during Fe(II) oxidation by the nitrate-reducing Acidovorax sp. strain BoFeN1 using complementary microscopy tomography approaches, Geobiology, 12, 340-361 (2014), online: 14.05.2014, doi:10.1111/gbi.12088 [Link]
Abstract:

The formation of cell-(iron)mineral aggregates as a consequence of bacterial iron oxidation is an environmen- tally widespread process with a number of implications for processes such as sorption and coprecipitation of contaminants and nutrients. Whereas the overall appearance of such aggregates is easily accessible using 2-D microscopy techniques, the 3-D and internal structure remain obscure. In this study, we examined the 3-D structure of cell-(iron)mineral aggregates formed during Fe(II) oxidation by the nitrate-reducing Acidovorax sp. strain BoFeN1 using a combination of advanced 3-D microscopy techniques. We obtained 3-D structural and chemical information on different cellular encrustation patterns at high spatial resolution (4–200 nm, depending on the method): more specifically, (1) cells free of iron minerals, (2) periplasm filled with iron minerals, (3) spike- or platelet-shaped iron mineral structures, (4) bulky structures on the cell surface, (5) extracellular iron mineral shell structures, (6) cells with iron mineral filled cytoplasm, and (7) agglomerations of extracellular globular structures. In addition to structural information, chemical nanotomography suggests a dominant role of extracellular polymeric substances (EPS) in controlling the formation of cell-(iron)mineral aggregates. Furthermore, samples in their hydrated state showed cell-(iron)mineral aggregates in pristine conditions free of preparation (i.e., drying/dehydration) artifacts. All these results were obtained using 3-D microscopy techniques such as focused ion beam (FIB)/scanning electron microscopy (SEM) tomography, transmission electron microscopy (TEM) tomography, scanning transmission (soft) X-ray microscopy (STXM) tomography, and confocal laser scanning microscopy (CLSM). It turned out that, due to the various different contrast mechanisms of the individual approaches, and due to the required sample preparation steps, only the combination of these techniques was able to provide a comprehensive understanding of structure and composition of the various Fe-precipitates and their association with bacterial cells and EPS.

Aktuelle Termine


BayCEER-Kolloquium:
Do. 12.12.2019
Iron, sulfur and a pinch of antimony - new perspectives on secondary mineral pathways and metalloid mobility
Geographisches Kolloquium:
Di. 10.12.2019
Intensify or diversify? How agriculture affects biodiversity and ecosystem processes in European farmland
Di. 17.12.2019
The meat of the Anthropocene: Food, capital and the globalisation of industrialised animal killing
Ökologisch-Botanischer Garten:
So. 05.01.2020
Auf ins Neue! Winterspaziergang im ÖBG
So. 05.01.2020
Konzert: Musikalischer Jahresbeginn mit den Rockin`Dinos
BayCEER Blog
24.05.2019
Stoichiometric controls of C and N cycling
07.05.2019
Flying halfway across the globe to dig in the dirt – a research stay in Bloomington, USA
07.05.2019
EGU – interesting research and free coffee
16.04.2019
Picky carnivorous plants?
RSS Blog als RSS Feed
Wetter Versuchsflächen
Luftdruck (356m): 968.0 hPa
Lufttemperatur: 7.3 °C
Niederschlag: 0.2 mm/24h
Sonnenschein: <1 h/d
Wind (Höhe 17m): 9.4 km/h
Wind (Max.): 20.2 km/h
Windrichtung: SW

...mehr
Globalstrahlung: 21 W/m²
Lufttemperatur: 3.5 °C
Niederschlag: 0.7 mm/24h
Sonnenschein: <1 h/d
Wind (Höhe 32m): 25.4 km/h

...mehr
Diese Webseite verwendet Cookies. weitere Informationen