Joens, MS; Huynh, C; Kasuboski, JM; Ferranti, D; Sigal, YJ; Zeitvogel, F; Obst, M; Burkhardt, CJ; Curran, KP; Chalasani, SH; Stern, LA; Goetze, B; Fitzpatrick, JAJ: Helium Ion Microscopy (HIM) for the imaging of biological samples at sub-nanometer resolution, Scientific Reports, 3(3514), 1-7 (2013), online: 17.12.2013, doi:10.1038/srep03514 [Link]
Abstract:

Scanning Electron Microscopy (SEM) has long been the standard in imaging the sub-micrometer surface ultrastructure of both hard and soft materials. In the case of biological samples, it has provided great insights into their physical architecture. However, three of the fundamental challenges in the SEM imaging of soft materials are that of limited imaging resolution at high magnification, charging caused by the insulating properties of most biological samples and the loss of subtle surface features by heavy metal coating. These challenges have recently been overcome with the development of the Helium Ion Microscope (HIM), which boasts advances in charge reduction, minimized sample damage, high surface contrast without the need for metal coating, increased depth of field, and 5 angstrom imaging resolution. We demonstrate the advantages of HIM for imaging biological surfaces as well as compare and contrast the effects of sample preparation techniques and their consequences on sub-nanometer ultrastructure.

Aktuelle Termine


Antrittsvorlesung:
Mi. 30.10.2019
Prof. Tillmann Lüders: Aktiv im Untergrund - die Bedeutung der Mikroben für die Grundwasserqualität
BayCEER-Kolloquium:
Do. 24.10.2019
Inside the fog: towards an improved forecasting of nocturnal fog by using turbulence-resolving simulations
Fr. 08.11.2019
Umweltseismologie und ihre Bedeutung zur Abschätzung geomorphologischer Risiken
Ökologisch-Botanischer Garten:
So. 03.11.2019
Bittersüßer Wohlgeschmack: "Kolonialpflanzen"
So. 03.11.2019
Ausstellungseröffnung: Farben-Pflanzen-Pflanzenfarben - Malerei von Angelika Gigauri
BayCEER Blog
24.05.2019
Stoichiometric controls of C and N cycling
07.05.2019
Flying halfway across the globe to dig in the dirt – a research stay in Bloomington, USA
07.05.2019
EGU – interesting research and free coffee
16.04.2019
Picky carnivorous plants?
RSS Blog als RSS Feed
Wetter Versuchsflächen
Luftdruck (356m): 975.0 hPa
Lufttemperatur: 11.4 °C
Niederschlag: 0.0 mm/24h
Sonnenschein: 3 h/d
Wind (Höhe 17m): 2.5 km/h
Wind (Max.): 4.3 km/h
Windrichtung: O

...mehr
Globalstrahlung: 72 W/m²
Lufttemperatur: 12.4 °C
Niederschlag: 0.2 mm/24h
Sonnenschein: <1 h/d
Wind (Höhe 32m): 4.3 km/h

...mehr
Diese Webseite verwendet Cookies. weitere Informationen