|Hunter, RC; Hitchcock, AP; Dynes, JJ; Obst, M; Beveridge, TJ: Mapping the Speciation of Iron in Pseudomonas aeruginosa Biofilms Using Scanning Transmission X-ray Microscopy, Environmental Science and Technology, 42(23), 8766-8772 (2008), online: 28.10.2008, doi:10.1021/es801642z [Link]|
An important feature of microbial communities is the spatial heterogeneity of extracellular chemistry. Scanning transmission X-ray microscopy (STXM) was used to map the spatial distribution of iron species throughout Pseudomonas aeruginosa biofilms to assess the influence of chemical heterogeneity on biomineralization. P. aeruginosa biofilms were treated with Fe(III)-amended media. Speciation and quantitative mapping using STXM image sequences in the Fe 2p3/2 (L3) absorption edge region revealed both Fe(II) and Fe(III) in localized microenvironments. Fe(III) was mainly associated with cell surfaces, while small amounts of Fe(II) was found in the extracellular space. Biofilms were also characterized using C 1s edge STXM image sequences. Anaerobic growth assays and confocal microscopy revealed the inability of P. aeruginosa to directly reduce Fe(III), implicating indirect iron reduction mechanisms in the formation of fine-grained, multivalent minerals. These studies suggest that geochemical microenvironments found throughout microbial communities are even more complex than previously believed.
Physical constraints and biological controls of plant-environment interactions
Presentations W1/W3 Professorship Geoinformatics and Spatial Big Data
Verschoben auf WS 2020/21! Investigating communal pathogen defense and its role in social evolution
|Mi. 01.07.2020 aktuell|
Alle Termine im Juli 2020 entfallen!
|Fr. 03.07.2020 aktuell|
Gewächshäuser ab sofort wieder geöffnet!
Why Science Communication?
Stoichiometric controls of C and N cycling
Flying halfway across the globe to dig in the dirt – a research stay in Bloomington, USA
EGU – interesting research and free coffee