Obst, M; Dittrich, M; Kuehn, H: Calcium adsorption and changes of the surface microtopography of cyanobacteria studied by AFM, CFM, and TEM with respect to biogenic calcite nucleation, Geochemistry, Geophysics, Geosystems, 7(6) (2006), online: 14.06.2006, doi:10.1029/2005GC001172 [Link]

We investigated calcium interactions with the surface of individual living cells of the freshwater cyanobacterium Synechococcus leopoliensis PCC 7942 under illumination and calcite supersaturation, with respect to calcium carbonate nucleation on the cell surface. Immobilized living cells were scanned by Atomic Force Microscopy (AFM) with conventional and chemically modified tips which were coated with carboxylic groups, so-called Chemical Force Microscopy (CFM). Calcium adsorption was heterogeneous and altered the surface charge of the cells. This was confirmed by bulk measurements of the zeta-potential at different Ca2+ concentrations and compared to measurements made with other bivalent cations (Mg2+, Sr2+, Ba2+). Zeta-potential increased rapidly (<2 min) from −46.9 mV in calcium-free solution to +0.8 mV in 22 mmol L−1 CaCl2 solution, using a 1.5 mmol L−1 NaHCO3 solution as background electrolyte in both cases. This was followed by the slow (several hours) formation of nonmineralized protuberances at the cell surface. These changes in the microtopography did not show obvious spatial relationship to surface charge or Ca2+ adsorption. The formation of the protuberances was reversible when Ca2+ was removed from the circumfluent solution. Ca2+ adsorption to the cell surface was faster than calcium-induced changes in the surface microtopography and much faster than calcite nucleation on the cell surface which was observed on a fraction of the cells in previous bulk experiments under similar supersaturation.

Aktuelle Termine
Ökologisch-Botanischer Garten:
Mi. 11.08.2021
Führung: "Grüne Apotheke: Heilpflanzen
So. 15.08.2021
Führung: Der ÖGB zum Kennenlernen
So. 05.09.2021
Führung "Lebensraum Wiese: Vielfalt erforschen"
Mi. 08.09.2021
Führung "Erdnuss und Johannisbrot: Tropische Hülsenfrüchte"
Mi. 15.09.2021
Führung "Authentische Einblicke in die Welt der Kaffeebauern - Führung durch die Ausstellung
Wetter Versuchsflächen
Luftdruck (356m): 971.0 hPa
Lufttemperatur: 14.3 °C
Niederschlag: 0.0 mm/24h
Sonnenschein: 1 h/d
Wind (Höhe 17m): 4.7 km/h
Wind (Max.): 12.2 km/h
Windrichtung: SW

Globalstrahlung: 45 W/m²
Lufttemperatur: 11.5 °C
Niederschlag: 0.6 mm/24h
Sonnenschein: <1 h/d
Wind (Höhe 32m): 0.0 km/h

Diese Webseite verwendet Cookies. weitere Informationen