|Zeitvogel, F; Burkhardt, CJ; Schroeppel, B; Schmid, G; Ingino, P; Obst, M: Comparison of Preparation Methods of Bacterial Cell-Mineral Aggregates for SEM Imaging and Analysis Using the Model System of Acidovorax sp. BoFeN1, Geomicrobiology Journal, 34(4), 317-327 (2017), online: 23.06.2016, doi:10.1080/01490451.2016.1189467 [Link]|
Innovative microanalytical methods are valuable tools in geomicrobiology. They often require the use of dried samples, demanding a challenging sample preparation. Since geomicrobiological samples typically have a strongly heterogeneous composition, choosing a preparation method is not straightforward. We therefore compared how different drying methods (critical point drying, hexamethyldisilazane drying, air drying and freeze drying) influence the structure of bacterial cell-mineral aggregates. Each method proved suitable for a specific purpose, but none was able to completely preserve the sample structure. Additional information was obtained on surface alterations by sputter coating and on preservation of extracellular polymeric substances during resin embedding.
|Fr. 10.07.2020 aktuell|
12th BayCEER Workshop 2020: "Call for Abstracts" geöffnet
Extreme redox oscillations in freshwater re-flooded acid sulfate soil wetlands: Effects on Fe, S, and trace metals geochemical behavior
Dissolved organic matter quality in differently managed forest ecosystems
Signaling of rhizosphere microbiome: key for plant health, development and nutrition
Neuer Termin: BayCEER Workshop 2020
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