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Ricke, P; Kolb, S; Braker, G: Application of a Newly Developed ARB Integrated in silico T-RFLP Tool Reveals the Importance of a Novel pmoA Sequence Cluster in an Upland Soil, Applied and Environmental Microbiology, 71(3), 1671-1673 (2005)
TRF-CUT, an ARB-implemented tool, was developed to predict in silico the terminal restriction fragments of aligned small-subunit rRNA gene or functional gene sequences. Application of this new tool to perform directed terminal restriction fragment length polymorphism analysis of pmoA products obtained from a forest soil revealed that novel cluster I methanotrophic bacteria were dominant.
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