| Liu, YJ; Liu, SJ; Drake, HL; Horn, MA: Consumers of 4-Chloro-2-Methylphenoxyacetic Acid from Agricultural Soil and Drilosphere Harbour cadA, r/sdpA, and tfdA-like Gene Encoded Oxygenases, FEMS Microbiology Ecology, 86, 114-129 (2013) | |
| Abstract: Microbial degradation of 2-Methyl-4-chlorophenoxyacetic acid (MCPA) in soil is enhanced by earthworms and intiated by tfdA-like, cadA and r/sdpA gene encoded oxygenases. Copy numbers of such genes increased during MCPA degradation in soil, and MCPA stimulated transcription of tfdA-like and r/sdpA genes up to 4x. Transcription of cadA was detected with MCPA only. DNA stable isotope probing after consumption of 0.6-0.8 mg 13C-MCPA gdw-1 in oxic microcosms indicated diverse labeled oxygenase genes in bulk soil, burrow walls and cast. 9, 6 and 3 operational taxonomic units of tfdA-like, cadA and r/sdpA genes, respectively, were labeled, and affiliated with group 2 Alphaproteobacteria including Bradyrhizobia and group 1 class III Betaproteobacteria. New genes encoding putative MCPA oxygenases were identified. Diversity of labeled OTUs tended to be lower for cast than for bulk soil. The collective data indicate (i) hitherto unknown active MCPA degraders and/or oxygenase genes in soil, (ii) that multiple oxygenases are associated with MCPA degradation in soil at the same time, (iii) that earthworms impact the capability of MCPA degraders in soil to respond to MCPA, and (iv) enables a more in depth analysis of MCPA degrader communities in soil by future structural gene based experimental strategies. |
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| BayCEER-Kolloquium: |
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| Do. 25.04.2024 Perspectives and challenges in the restoration and conservation of two isolated habitats: gypsum and cliffs |
| BayCEER Short Courses: |
| Mi. 24.04.2024 Mobile Film Making Workshop (for PhDs/PostDosc/Profs of BayCEER) |
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