| Kuhner, CH; Grießhammer, A; Drake, HL; Frank, C; Schmittroth, M; Acker, G; Gößner, A: Sporomusa silvacetica sp. nov., an acetogenic bacterium isolated from aggregated forest soil, Int. J. Syst. Bacteriol., 47, 352-358 (1997) | |
| Abstract: Sporomusa silvacetica sp. nov. strain DSM 10669T (T = type strain) was isolated from well-drained, aggregated forest soil (pH 6.0) in east-central Germany. Cells were obligately anaerobic, slightly curved rods, and motile with laterally-inserted flagella on the concave side of the cell. Typical cells were approximately 3.5 x 0.7 µm. Cells stained weakly Gram-positive, but thin sections displayed a complex multilayered cell wall. Spores were spherical and distended the sporangium. Growth and substrate utilization occurred with ferulate, vanillate, fructose, betaine, fumarate, 2,3-butanediol, pyruvate, lactate, glycerol, ethanol, methanol, formate, and H2-CO2. With most substrates, acetate was the primary reduced end-product and was produced in stoichiometries indicative of an acetyl-CoA pathway-dependent metabolism. Fumarate was dismutated to succinate and acetate. Methoxyl and acrylate groups of various aromatic compounds were O-demethylated and reduced, respectively. Yeast extract was not required for growth. Cells grew optimally at approximately 30°C and pH 6.8; under these conditions and with fructose as substrate, the doubling time approximated 14 h. The lowest temperature supporting growth was between 5-10°C. Carbon monoxide dehydrogenase and hydrogenase activities approximated 9 and 102 µmol min-1 mg-1 protein, respectively. A b-type cytochrome was detected in the membrane. The G+C content was approximately 43 mol %. Phylogenetic analysis of 16S rDNA indicated that DG-1 was most closely related to members of the genus Sporomusa within the Clostridium subphylum of the Gram-positive bacteria. |
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