Wiesenberg, GLB; Gocke, M; Kuzyakov, Y: Optimization of 14C liquid scintillation counting of plant and soil lipids to trace short term formation, translocation and degradation of lipids, Journal of Radioanalytical and Nuclear Chemistry, 284(1), 99-108 (2010), doi:10.1007/s10967-010-0450-7
Two powerful approaches are frequently used to trace incorporation and degradation of plant derived C in soil: 14C labelling/chasing and analysis of lipid composition. In this study, we coupled these approaches in order to trace short term incorporation of plant derived lipids into rhizosphere and non-rhizosphere soil. Methodological optimization was required and implied 14C liquid scintillation counting improvement for plant lipid extracts taking into account organic solvents, solvent-to-scintillation cocktail ratio, and amount of lipids. Following method optimization, 14C data of fatty acids indicated a notable contribution of root derived lipids to rhizosphere and nonrhizosphere soil. Coupling of 14C labelling/chasing with lipid analysis is a powerful and cheap approach for tracing of root derived C in soil allowing for estimation of C budget, for determination of C formation and translocation within plants and from plant to soil, as well as for identification of short term dynamics of specific compound classes within soil.
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