Wiesenberg, GLB; Gocke, M; Kuzyakov, Y: Optimization of 14C liquid scintillation counting of plant and soil lipids to trace short term formation, translocation and degradation of lipids, Journal of Radioanalytical and Nuclear Chemistry, 284(1), 99-108 (2010), doi:10.1007/s10967-010-0450-7
Abstract:
Two powerful approaches are frequently used to trace incorporation and degradation of plant derived C in soil: 14C labelling/chasing and analysis of lipid composition. In this study, we coupled these approaches in order to trace short term incorporation of plant derived lipids into rhizosphere and non-rhizosphere soil. Methodological optimization was required and implied 14C liquid scintillation counting improvement for plant lipid extracts taking into account organic solvents, solvent-to-scintillation cocktail ratio, and amount of lipids. Following method optimization, 14C data of fatty acids indicated a notable contribution of root derived lipids to rhizosphere and nonrhizosphere soil. Coupling of 14C labelling/chasing with lipid analysis is a powerful and cheap approach for tracing of root derived C in soil allowing for estimation of C budget, for determination of C formation and translocation within plants and from plant to soil, as well as for identification of short term dynamics of specific compound classes within soil.
Aktuelle Termine


BayCEER-Kolloquium:
Do. 09.12.2021
Understanding mycorrhizal functions across scales
Do. 16.12.2021
Abschiedsvorlesung: Wozu lassen sich Modelle von Ökosystemen verwenden?
Digitale Vortragsreihe KlimaDiskurse (bayklif):
Di. 14.12.2021
Tropisches Eis? Die Macht der Kunst im Kampf um 1.5°
Di. 11.01.2022
Wie gelingt die Energiewende? Soziale Innovationen als Motor der Transformation
Ökologisch-Botanischer Garten:
So. 02.01.2022
Führung | Mit kühlem Kopf ins Neue: Winterspaziergang
Wetter Versuchsflächen
Luftdruck (356m): 959.1 hPa
Lufttemperatur: 0.2 °C
Niederschlag: 1.0 mm/24h
Sonnenschein: 4 h/d
Wind (Höhe 17m): 8.0 km/h
Wind (Max.): 19.4 km/h
Windrichtung: SO

...mehr
Globalstrahlung: 79 W/m²
Lufttemperatur: -2.1 °C
Niederschlag: 0.1 mm/24h
Sonnenschein: <1 h/d
Wind (Höhe 32m): 0.0 km/h

...mehr
Diese Webseite verwendet Cookies. weitere Informationen