Nitrogen dioxide (NO₂) plays an important role in atmospheric pollution, in particular for tropospheric ozone production. However, the removal processes involved in NO₂ deposition to terrestrial ecosystems are still subject of ongoing discussion. This study reports NO₂ flux measurements made over a meadow using the eddy covariance method. The measured NO₂ deposition fluxes during daytime were about a factor of two lower than a priori calculated fluxes using the Surfatm model without taking into account an internal (also called mesophyllic or sub-stomatal) resistance. Neither an underestimation of the measured NO2 deposition flux due to chemical divergence or direct NO₂ emission, nor an underestimation of the resistances used to model the NO₂ deposition explained the large difference between measured and modelled NO2 fluxes. Thus, only the existence of the internal resistance could account for this large discrepancy between model and measurements. The median internal resistance was estimated to 300 s m⁻¹ during daytime, but exhibited a large variability (100 s m⁻¹ to 800 s m⁻¹). In comparison, the stomatal resistance was only around 100 s m⁻¹ during daytime. Hence, the internal resistance accounted for 50% to 90% of the total leaf resistance to NO₂. This study presents the first clear evidence and quantification of the internal resistance using the eddy covariance method, i.e. plant functioning was not affected by changes of microclimatological (turbulent) conditions that typically occur when using enclosure methods.