Zeitvogel, F; Burkhardt, CJ; Schroeppel, B; Schmid, G; Ingino, P; Obst, M: Comparison of Preparation Methods of Bacterial Cell-Mineral Aggregates for SEM Imaging and Analysis Using the Model System of Acidovorax sp. BoFeN1, Geomicrobiology Journal, 34(4), 317-327 (2017), online: 2016-06-23, doi:10.1080/01490451.2016.1189467 [Link]
Abstract:
Innovative microanalytical methods are valuable tools in geomicrobiology. They often require the use of dried samples, demanding a challenging sample preparation. Since geomicrobiological samples typically have a strongly heterogeneous composition, choosing a preparation method is not straightforward. We therefore compared how different drying methods (critical point drying, hexamethyldisilazane drying, air drying and freeze drying) influence the structure of bacterial cell-mineral aggregates. Each method proved suitable for a specific purpose, but none was able to completely preserve the sample structure. Additional information was obtained on surface alterations by sputter coating and on preservation of extracellular polymeric substances during resin embedding.
Upcoming ...


BayCEER Colloquium:
Th. 2021-01-14
Mineral surfaces and organic matter accrual in soils
Th. 2021-01-21
The impact of trees on soil organic carbon dynamics in the Subarctic - Priming effects and microbial N mining
Ecological-Botanical Garden:
Su. 2020-12-06
Entfällt: Führung nach Anmeldung: Zimt & Mandelkern: Pflanzen in der Weihnachtsbäckerei
Su. 2021-01-03
Führung nach Anmeldung: Mit kühlem Kopf ins Neue: Winterspaziergang
Geographisches Kolloquium:
Tu. 2020-12-08
People, pathogens, places: where medical geography meets disease ecology?
BayCEER Blog
13.01.2020
Why Science Communication?
24.05.2019
Stoichiometric controls of C and N cycling
07.05.2019
Flying halfway across the globe to dig in the dirt – a research stay in Bloomington, USA
16.04.2019
Picky carnivorous plants?
RSS Blog as RSS Feed
Weather research site
Luftdruck (356m): 948.3 hPa
Lufttemperatur: 2.6 °C
Niederschlag: 0.0 mm/24h
Sonnenschein: <1 h/d
Wind (Höhe 17m): 16.0 km/h
Wind (Max.): 35.3 km/h
Windrichtung: SO

...more
Globalstrahlung: -2 W/m²
Lufttemperatur: -2.5 °C
Niederschlag: 0.0 mm/24h
Sonnenschein: <1 h/d
Wind (Höhe 32m): 0.0 km/h

...more
This site makes use of cookies More information