Halimaa, P; Lin, Y-F; Ahonen, VH; Blande, D; Clemens, S; Gyenesei, A; Häikiö, E; Kärenlampi, SO; Laiho, A; Aarts, MGM; Pursiheimo, J-P; Schat, H; Schmidt, H; Tuomainen, MH; Tervahauta, AI: Gene expression differences between Noccaea caerulescens ecotypes help identifying candidate genes for metal phytoremediation., Environmental Science Technology (2014), doi:10.1021/es4042995 | |
Abstract: Populations of Noccaea caerulescens show tremendous differences in their capacity to hyperaccumulate and hypertolerate metals. To explore the differences that could contribute to these traits, we undertook SOLiD high-throughput sequencing of the root transcriptomes of three phenotypically well-characterized N. caerulescens accessions, i.e., Ganges, La Calamine, and Monte Prinzera. Genes with possible contribution to zinc, cadmium, and nickel hyperaccumulation and hypertolerance were predicted. The most significant differences between the accessions were related to metal ion (di-, trivalent inorganic cation) transmembrane transporter activity, iron and calcium ion binding, (inorganic) anion transmembrane transporter activity, and antioxidant activity. Analysis of correlation between the expression profile of each gene and the metal-related characteristics of the accessions disclosed both previously characterized (HMA4, HMA3) and new candidate genes (e.g., for nickel IRT1, ZIP10, and PDF2.3) as possible contributors to the hyperaccumulation/tolerance phenotype. A number of unknown Noccaea-specific transcripts also showed correlation with Zn(2+), Cd(2+), or Ni(2+) hyperaccumulation/tolerance. This study shows that N. caerulescens populations have evolved great diversity in the expression of metal-related genes, facilitating adaptation to various metalliferous soils. The information will be helpful in the development of improved plants for metal phytoremediation. |
BayCEER Short Courses: |
---|
Fr. 26.04.2024 aktuell Mobile Film Making Workshop (for students of BayCEER) |
Ökologisch-Botanischer Garten: |
So. 28.04.2024 Führung | Den Sängern auf der Spur: Vogelstimmen im ÖBG (zus. mit LBV) |
So. 05.05.2024 Führung | Faszination Englische Gärten: Gestaltung und Pflanzen |
So. 05.05.2024 Midissage | da capo - Ausstellung von Doris Bocka |
Mi. 15.05.2024 Kurzführung | "Botanische Mittagspause" |