| Böttcher, C; v. Roepenack-Lahaye, E; Schmidt, J; Clemens, S; Scheel, D: Analysis of Phenolic Choline Esters from Seeds of Arabidopsis thaliana and Brassica napus by Capillary Liquid Chromatography/Electrospray-Tandem Mass Spectrometry, , J. Mass Spectrom. 44, 466-476 (2009), doi:10.1002/jms.1522 | |
| Abstract: Total phenolic choline ester fractions prepared from seeds of Arabidopsis thaliana and Brassica napus were analyzed by capillary LC/ESI-QTOF-MS and direct infusion ESI-FTICR-MS. In addition to the dominating sinapoylcholine, 30 phenolic choline esters could be identified based on accurate mass measurements, interpretation of collision-induced dissociation (CID) mass spectra, and synthesis of selected representatives. The compounds identified so far include substituted hydroxycinnamoyl- and hydroxybenzoylcholines, respective monohexosides as well as oxidative coupling products of phenolic choline esters and monolignols. Phenolic choline esters are well separable by reversed-phase liquid chromatography and sensitively detectable using electrospray ionization mass spectrometry in positive ion mode. CID mass spectra obtained from molecular ions facilitate the characterization of both the type and substitution pattern of such compounds. Therefore, LC/ESI-MS/MS represents a valuable tool for comprehensive qualitative and quantitative analysis of this compound class. |
Aktuelle Termine
Aktuelle Termine
| BayCEER Short Courses: |
|---|
| Fr. 26.04.2024 aktuell Mobile Film Making Workshop (for students of BayCEER) |
| Ökologisch-Botanischer Garten: |
| So. 28.04.2024 Führung | Den Sängern auf der Spur: Vogelstimmen im ÖBG (zus. mit LBV) |
| So. 05.05.2024 Führung | Faszination Englische Gärten: Gestaltung und Pflanzen |
| So. 05.05.2024 Midissage | da capo - Ausstellung von Doris Bocka |
| Mi. 15.05.2024 Kurzführung | "Botanische Mittagspause" |